abstract：BACKGROUND:Sesquiterpenes are a class of natural products with a diverse range of attractive industrial proprieties. Due to economic difficulties of sesquiterpene production via extraction from plants or chemical synthesis there is interest in developing alternative and cost efficient bioprocesses. The hydrocarbon α-sa...

journal_title：Microbial cell factories

authors： Scalcinati G,Partow S,Siewers V,Schalk M,Daviet L,Nielsen J

更新日期：2012-08-31 00:00:00

abstract：BACKGROUND:The understanding of the molecular basis of yeast tolerance to ethanol may guide the design of rational strategies to increase process performance in industrial alcoholic fermentations. A set of 21 genes encoding multidrug transporters from the ATP-Binding Cassette (ABC) Superfamily and Major Facilitator Sup...

journal_title：Microbial cell factories

authors： Teixeira MC,Godinho CP,Cabrito TR,Mira NP,Sá-Correia I

更新日期：2012-07-27 00:00:00

abstract：BACKGROUND:Prions were first identified as infectious proteins associated with fatal brain diseases in mammals. However, fungal prions behave as epigenetic regulators that can alter a range of cellular processes. These proteins propagate as self-perpetuating amyloid aggregates being an example of structural inheritance...

journal_title：Microbial cell factories

authors： Espargaró A,Villar-Piqué A,Sabaté R,Ventura S

更新日期：2012-06-25 00:00:00

abstract：:A growing number of insights on the biology of bacterial inclusion bodies (IBs) have revealed intriguing utilities of these protein particles. Since they combine mechanical stability and protein functionality, IBs have been already exploited in biocatalysis and explored for bottom-up topographical modification in tiss...

journal_title：Microbial cell factories

authors： Villaverde A,García-Fruitós E,Rinas U,Seras-Franzoso J,Kosoy A,Corchero JL,Vazquez E

更新日期：2012-06-11 00:00:00

abstract：BACKGROUND:Lipases (EC 3.1.1.3) catalyze the hydrolysis of triacyl glycerol to glycerol and are involved in the synthesis of both short chain and long chain acylglycerols. They are widely used industrially in various applications, such as baking, laundry detergents and as biocatalysts in alternative energy strategies. ...

journal_title：Microbial cell factories

authors： Selvin J,Kennedy J,Lejon DP,Kiran GS,Dobson AD

更新日期：2012-06-01 00:00:00

abstract：BACKGROUND:The E. coli lac operon and its components have been studied for decades, and lac-derived systems are widely used for recombinant protein production. However, lac operon dynamics and induction behavior remain the paradigm of gene regulation. Recently, an HPLC-MS-based method to quantify IPTG in the medium and...

journal_title：Microbial cell factories

authors： Fernández-Castané A,Caminal G,López-Santín J

更新日期：2012-05-09 00:00:00

abstract：BACKGROUND:2'-Fucosyllactose (2-FL) is a functional oligosaccharide present in human milk which protects against the infection of enteric pathogens. Because 2-FL can be synthesized through the enzymatic fucosylation of lactose with guanosine 5'-diphosphate (GDP)-l-fucose by α-1,2-fucosyltransferase (FucT2), an 2-FL pro...

journal_title：Microbial cell factories

authors： Lee WH,Pathanibul P,Quarterman J,Jo JH,Han NS,Miller MJ,Jin YS,Seo JH

更新日期：2012-04-30 00:00:00

abstract：BACKGROUND:L-tryptophan is an aromatic amino acid widely used in the food, chemical and pharmaceutical industries. In Escherichia coli, L-tryptophan is synthesized from phosphoenolpyruvate and erythrose 4-phosphate by enzymes in the shikimate pathway and L-tryptophan branch pathway, while L-serine and phosphoribosylpyr...

journal_title：Microbial cell factories

authors： Gu P,Yang F,Kang J,Wang Q,Qi Q

更新日期：2012-03-02 00:00:00

abstract：BACKGROUND:Fumaric acid is a commercially important component of foodstuffs, pharmaceuticals and industrial materials, yet the current methods of production are unsustainable and ecologically destructive. RESULTS:In this study, the fumarate biosynthetic pathway involving reductive reactions of the tricarboxylic acid c...

journal_title：Microbial cell factories

authors： Xu G,Liu L,Chen J

更新日期：2012-02-15 00:00:00

abstract：BACKGROUND:Molecular mechanisms generating genetic variation provide the basis for evolution and long-term survival of a population in a changing environment. In stable, laboratory conditions, the variation-generating mechanisms are dispensable, as there is limited need for the cell to adapt to adverse conditions. In f...

journal_title：Microbial cell factories

authors： Csörgo B,Fehér T,Tímár E,Blattner FR,Pósfai G

更新日期：2012-01-20 00:00:00

abstract：BACKGROUND:Cellobiose dehydrogenase (CDH) is an extracellular hemoflavoenzyme produced by lignocellulose-degrading fungi including Pycnoporus cinnabarinus. We investigated the cellulolytic system of P. cinnabarinus, focusing on the involvement of CDH in the deconstruction of lignocellulosic biomass. RESULTS:First, P. ...

journal_title：Microbial cell factories

authors： Bey M,Berrin JG,Poidevin L,Sigoillot JC

更新日期：2011-12-28 00:00:00

abstract：BACKGROUND:Recombinant antibodies can be produced in different formats and different expression systems. Single chain variable fragments (scFvs) represent an attractive alternative to full-length antibodies and they can be easily produced in bacteria or yeast. However, the scFvs exhibit monovalent antigen-binding prope...

journal_title：Microbial cell factories

authors： Pleckaityte M,Zvirbliene A,Sezaite I,Gedvilaite A

更新日期：2011-12-15 00:00:00

abstract：BACKGROUND:Bacterial surface display is of interest in many applications, including live vaccine development, screening of protein libraries and the development of whole cell biocatalysts. The goal of this work was to understand which parameters result in production of large quantities of cells that at the same time ex...

journal_title：Microbial cell factories

authors： Gustavsson M,Bäcklund E,Larsson G

更新日期：2011-09-14 00:00:00

abstract：BACKGROUND:A stereoisomer of inositol, scyllo-inositol, is known as a promising therapeutic agent for Alzheimer's disease, since it prevents the accumulation of beta-amyloid deposits, a hallmark of the disease. However, this compound is relatively rare in nature, whereas another stereoisomer of inositol, myo-inositol, ...

journal_title：Microbial cell factories

authors： Yamaoka M,Osawa S,Morinaga T,Takenaka S,Yoshida K

更新日期：2011-09-07 00:00:00

abstract：BACKGROUND:Lactobacillus ruminis is a poorly characterized member of the Lactobacillus salivarius clade that is part of the intestinal microbiota of pigs, humans and other mammals. Its variable abundance in human and animals may be linked to historical changes over time and geographical differences in dietary intake of...

journal_title：Microbial cell factories

authors： O'Donnell MM,Forde BM,Neville B,Ross PR,O'Toole PW

更新日期：2011-08-30 00:00:00

abstract：BACKGROUND:Most bacteria can use various compounds as carbon sources. These carbon sources can be either co-metabolized or sequentially metabolized, where the latter phenomenon typically occurs as catabolite repression. From the practical application point of view of utilizing lignocellulose for the production of biofu...

journal_title：Microbial cell factories

authors： Yao R,Hirose Y,Sarkar D,Nakahigashi K,Ye Q,Shimizu K

更新日期：2011-08-11 00:00:00

abstract：BACKGROUND:The budding yeast Pichia pastoris is widely used for protein production. To determine the best suitable strategy for strain improvement, especially for high secretion, quantitative data of intracellular fluxes of recombinant protein are very important. Especially the balance between intracellular protein for...

journal_title：Microbial cell factories

authors： Pfeffer M,Maurer M,Köllensperger G,Hann S,Graf AB,Mattanovich D

更新日期：2011-06-26 00:00:00

abstract：BACKGROUND:L-arabinose is an important intermediate for anti-virus drug synthesis and has also been used in food additives for diets-controlling in recent years. Commercial production of L-arabinose is a complex progress consisting of acid hydrolysis of gum arabic, followed by multiple procedures of purification, thus ...

journal_title：Microbial cell factories

authors： Cheng H,Wang H,Lv J,Jiang M,Lin S,Deng Z

更新日期：2011-06-07 00:00:00

abstract：BACKGROUND:Triacylglycerols are used in various purposes including food applications, cosmetics, oleochemicals and biofuels. Currently the main sources for triacylglycerol are vegetable oils, and microbial triacylglycerol has been suggested as an alternative for these. Due to the low production rates and yields of micr...

journal_title：Microbial cell factories

authors： Santala S,Efimova E,Kivinen V,Larjo A,Aho T,Karp M,Santala V

更新日期：2011-05-18 00:00:00

abstract：BACKGROUND:Thymosin α1 (Tα1), a 28-amino acid Nα-acetylated peptide, has a powerful general immunostimulating activity. Although biosynthesis is an attractive means of large-scale manufacture, to date, Tα1 can only be chemosynthesized because of two obstacles to its biosynthesis: the difficulties in expressing small pe...

journal_title：Microbial cell factories

authors： Ren Y,Yao X,Dai H,Li S,Fang H,Chen H,Zhou C

更新日期：2011-04-22 00:00:00

abstract：BACKGROUND:Pichia pastoris is one of the most important host organisms for the recombinant production of proteins in industrial biotechnology. To date, strain specific parameters, which are needed to set up feeding profiles for fed batch cultivations, are determined by time-consuming continuous cultures or consecutive ...

journal_title：Microbial cell factories

authors： Dietzsch C,Spadiut O,Herwig C

更新日期：2011-03-03 00:00:00

abstract：BACKGROUND:Variations in codon usage between species are one of the major causes affecting recombinant protein expression levels, with a significant impact on the economy of industrial enzyme production processes. The use of codon-optimized genes may overcome this problem. However, designing a gene for optimal expressi...

journal_title：Microbial cell factories

authors： Menzella HG

更新日期：2011-03-03 00:00:00

abstract：BACKGROUND:In recent years, it has been gradually realized that bacterial inclusion bodies (IBs) could be biologically active. In particular, several proteins including green fluorescent protein, β-galactosidase, β-lactamase, alkaline phosphatase, D-amino acid oxidase, polyphosphate kinase 3, maltodextrin phosphorylase...

journal_title：Microbial cell factories

authors： Wu W,Xing L,Zhou B,Lin Z

更新日期：2011-02-15 00:00:00

abstract：BACKGROUND:Terpenoids constitute a large family of natural products, attracting commercial interest for a variety of uses as flavours, fragrances, drugs and alternative fuels. Saccharomyces cerevisiae offers a versatile cell factory, as the precursors of terpenoid biosynthesis are naturally synthesized by the sterol bi...

journal_title：Microbial cell factories

authors： Ignea C,Cvetkovic I,Loupassaki S,Kefalas P,Johnson CB,Kampranis SC,Makris AM

更新日期：2011-01-28 00:00:00

abstract：:Like natural viruses, manmade protein cages for drug delivery are to be ideally formed by repetitive subunits with self-assembling properties, mimicking viral functions and molecular organization. Naturally formed nanostructures (such as viruses, flagella or simpler protein oligomers) can be engineered to acquire spec...

journal_title：Microbial cell factories

authors： Vázquez E,Villaverde A

更新日期：2010-12-30 00:00:00

abstract：BACKGROUND:The efficiency of biotechnological production processes depends on selecting the best performing microbial strain and the optimal cultivation conditions. Thus, many experiments have to be conducted, which conflicts with the demand to speed up drug development processes. Consequently, there is a great need fo...

journal_title：Microbial cell factories

authors： Funke M,Buchenauer A,Mokwa W,Kluge S,Hein L,Müller C,Kensy F,Büchs J

更新日期：2010-11-13 00:00:00

abstract：BACKGROUND:Acetic acid is a byproduct of Saccharomyces cerevisiae alcoholic fermentation. Together with high concentrations of ethanol and other toxic metabolites, acetic acid may contribute to fermentation arrest and reduced ethanol productivity. This weak acid is also a present in lignocellulosic hydrolysates, a high...

journal_title：Microbial cell factories

authors： Mira NP,Palma M,Guerreiro JF,Sá-Correia I

更新日期：2010-10-25 00:00:00

abstract：BACKGROUND:To reduce the production cost of bioethanol obtained from fermentation of the sugars provided by degradation of lignocellulosic biomass (i.e., second generation bioethanol), it is necessary to screen for new enzymes endowed with more efficient biomass degrading properties. This demands the set-up of high-thr...

journal_title：Microbial cell factories

authors： Navarro D,Couturier M,da Silva GG,Berrin JG,Rouau X,Asther M,Bignon C

更新日期：2010-07-16 00:00:00

abstract：BACKGROUND:Recent years have seen a huge growth in the market of industrial yeasts with the need for strains affording better performance or to be used in new applications. Stress tolerance of commercial Saccharomyces cerevisiae yeasts is, without doubt, a trait that needs improving. Such trait is, however, complex, an...

journal_title：Microbial cell factories

authors： Pérez-Torrado R,Panadero J,Hernández-López MJ,Prieto JA,Randez-Gil F

更新日期：2010-07-13 00:00:00

abstract：:Nanotechnology is increasingly using both materials and nano-objects synthesized by living beings, most of them produced by microbial cells. Emerging technologies and highly integrative approaches (such as 'omics and systems biology), that have been largely proven successful for the production of proteins and secondar...

journal_title：Microbial cell factories

authors： Villaverde A

更新日期：2010-07-05 00:00:00

abstract：BACKGROUND:Recombinant protein production is universally employed as a solution to obtain the milligram to gram quantities of a given protein required for applications as diverse as structural genomics and biopharmaceutical manufacture. Yeast is a well-established recombinant host cell for these purposes. In this study...

journal_title：Microbial cell factories

authors： Ferndahl C,Bonander N,Logez C,Wagner R,Gustafsson L,Larsson C,Hedfalk K,Darby RA,Bill RM

更新日期：2010-06-17 00:00:00

abstract：BACKGROUND:The filamentous fungus T. reesei effectively degrades cellulose and is known to produce various cellulolytic enzymes such as beta-glucosidase, endoglucanase, and cellobiohydrolase. The expression levels of each cellulase are controlled simultaneously, and their ratios and synergetic effects are important for...

journal_title：Microbial cell factories

authors： Yamada R,Taniguchi N,Tanaka T,Ogino C,Fukuda H,Kondo A

更新日期：2010-05-14 00:00:00

abstract：BACKGROUND:The selection of bacterial recombinants that harbour a desired insert, has been a key factor in molecular cloning and a series of screening procedures need to be performed for selection of clones carrying the genes of interest. The conventional cloning techniques are reported to have problems such as screeni...

journal_title：Microbial cell factories

authors： Banerjee S,Kumar J,Apte-Deshpande A,Padmanabhan S

更新日期：2010-05-11 00:00:00

abstract：BACKGROUND:In spite of the substantial metabolic engineering effort previously devoted to the development of Saccharomyces cerevisiae strains capable of fermenting both the hexose and pentose sugars present in lignocellulose hydrolysates, the productivity of reported strains for conversion of the naturally most abundan...

journal_title：Microbial cell factories

authors： Krahulec S,Petschacher B,Wallner M,Longus K,Klimacek M,Nidetzky B

更新日期：2010-03-10 00:00:00

abstract：BACKGROUND:It is quite important to understand how the central metabolism is regulated under nitrogen (N)- limitation as well as carbon (C)- limitation. In particular, the effect of C/N ratio on the metabolism is of practical interest for the heterologous protein production, PHB production, etc. Although the carbon and...

journal_title：Microbial cell factories

authors： Kumar R,Shimizu K

更新日期：2010-01-27 00:00:00

abstract：BACKGROUND:Zygosaccharomyces bailii is a diploid budding yeast still poorly characterized, but widely recognised as tolerant to several stresses, most of which related to industrial processes of production. Because of that, it would be very interesting to develop its ability as a cell factory. Gas1p is a beta-1,3-gluca...

journal_title：Microbial cell factories

authors： Passolunghi S,Riboldi L,Dato L,Porro D,Branduardi P

更新日期：2010-01-26 00:00:00

abstract：BACKGROUND:Increasingly lignocellulosic biomass hydrolysates are used as the feedstock for industrial fermentations. These biomass hydrolysates are complex mixtures of different fermentable sugars, but also inhibitors and salts that affect the performance of the microbial production host. The performance of six industr...

journal_title：Microbial cell factories

authors： Rumbold K,van Buijsen HJ,Overkamp KM,van Groenestijn JW,Punt PJ,van der Werf MJ

更新日期：2009-12-04 00:00:00

abstract：:The first genome sequences of the important yeast protein production host Pichia pastoris have been released into the public domain this spring. In order to provide the scientific community easy and versatile access to the sequence, two web-sites have been installed as a resource for genomic sequence, gene and protein...

journal_title：Microbial cell factories

authors： Mattanovich D,Callewaert N,Rouzé P,Lin YC,Graf A,Redl A,Tiels P,Gasser B,De Schutter K

更新日期：2009-10-16 00:00:00

abstract：BACKGROUND:The expression of heterologous proteins in Escherichia coli is strongly affected by codon bias. This phenomenon occurs when the codon usage of the mRNA coding for the foreign protein differs from that of the bacterium. The ribosome pauses upon encountering a rare codon and may detach from the mRNA, thereby t...

journal_title：Microbial cell factories

authors： Rosano GL,Ceccarelli EA

更新日期：2009-07-24 00:00:00

abstract：BACKGROUND:The production of high yields of recombinant proteins is an enduring bottleneck in the post-genomic sciences that has yet to be addressed in a truly rational manner. Typically eukaryotic protein production experiments have relied on varying expression construct cassettes such as promoters and tags, or cultur...

journal_title：Microbial cell factories

authors： Bonander N,Darby RA,Grgic L,Bora N,Wen J,Brogna S,Poyner DR,O'Neill MA,Bill RM

更新日期：2009-01-29 00:00:00